投稿分類 微生物

主委發表種類: 壁報

投稿標題(中): 異硫氰酸酯和螯合劑合併使用於清除化學誘導持久細胞的評估

投稿標題(英): Evaluation of combined use of isothiocyanates and chelating agents to eradicate chemically induced persistent cells

投稿摘要: Abstract
Background&Objective: Infections with multidrug-resistant (MDR) bacteria is a serious problem for public health, more development of a new class of antibiotic and effective use of antibacterial agent is essential. Persister bacteria, characterized by a dormant-like state with reduced metabolic activity and elevated resistance to antibiotics, are phenotypically distinct but genetically identical to the rest of the bacterial population. Persistent bacteria are one of the key reasons for the recurrence of chronic infections. Isothiocyanates (ITCs) are natural antibacterial molecules that effectively inhibit the growth of various pathogens; EDTA is a chelating agent which has been shown to increase the permeability of bacterial outer membrane. We aimed to evaluate the synergistic action of combined use of EDTA with ITCs to fight against bacterial persister and inhibit the bacterial biofilm formation.
Method: Persister cells were enriched by chemical-induced method by exposure of bacteria to CCCP. The susceptibility of bacteria to antimicrobial agents were determined by minimal inhibitory concentration (MIC). The survival rate of persister cells was evaluated by exposure of bacteria to sub-MIC of ITCs in the presence or absence of EDTA.
Results: The number of persistent cells of Pseudomonas aeruginosa strain induced by CCCP was 100 times higher than that of untreated bacteria. There was no obvious difference in MIC values of antimicrobial compounds between CCCP-treated and CCCP-untreated bacteria. Meanwhile the survival number of persistent cells was significantly reduced by the combined use of sub-MIC of benzyl isothiocyanate (BITC) or indole-3-carbinol (I3C) with EDTA in a dose- and time-dependent mannered.
Conclusion: Our results indicated the combined use of ITCs with EDTA effectively eradicated the CCCP-induced persister cells that provided a strategy to fight against bacterial persister that may help to prevent the recurrence of bacterial infection.

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