投稿分類 其他

主委發表種類: 壁報

投稿標題(中): 台灣懷孕婦女乙型鏈球菌帶菌與血清型分析

投稿標題(英): Prevalence and serotype distribution of Streptococcus agalactiae colonization amongst pregnant women in Taiwan

投稿摘要: 前言
Group B streptococcus (GBS), also known as Streptococcus agalactiae, is a prevailing pathogen that leads to neonatal bacteremia, sepsis, meningitis, and even mortality. The study was aimed to investigate the antennal GBS colonization in pregnant women at the gestational age of 35-37 weeks in Taiwan, including prevalence, serotype distribution, antimicrobial resistance profiles, and molecular characters
方法
A total of 957 vaginal swabs were collected throughout the 2017 calendar year at one regional hospital in Changhua, Taiwan. GBS was isolated by (methods) and all isolates were tested for capsule serotypes by using multiplex polymerase chain reaction (PCR) assay. Multilocus sequence typing (MLST) was carried out on selective representative isolates. Antimicrobial susceptibility profile to penicillin, ampicillin, erythromycin, clindamycin, levofloxacin, and ceftriaxone was determined for each isolate using disk diffusion method in accordance with CLSI guideline. For isolates classified as clonal complex (CC) 17, PCR targeting the major virulence gene, HvgA, was performed.
討論
Of the 957 swab cultures, 218 were positive for GBS (22%). In total, seven serotypes were identified, and serotype III was the leading capsular type (25.2%) followed by VI (22.5%), V (14.7%), Ia (13.8%), II (11.9%), Ib (8.3%), VII (2.3%), VIII (0.5%) and NT (0.9%), respectively. Serotype IV was not detected in the present study. MLST analysis was carried out in 105 isolates and depicted 14 sequence types (ST). The leading four sequence types in order were ST1 (51, 48.6%), ST12 (9, 8.6%), ST17 (8, 7.6%) and ST890 (6, 5.7%). Of 105 MLST tested isolates, 21 were serotype VI and fully were ST1 (100%). For ST12, serotype II was the dominant type (9/10, 90%). In addition, a total of 8 isolates were identified as the hypervirulent CC17 strain and all were serotype III. All these 8 ST17-III isolates harbored HvgA gene. Four clonal complexes were found in this study and the predominant clonal complexes were CC1 (51, 48.6%), followed by CC23 (19, 18.1%). Regarding of antimicrobial resistance, all the isolates were susceptible to penicillin (100%). Resistance rates of erythromycin and clindamycin were 41.1% and 39.3%, respectively.
結論
In this present study, GBS colonization rate of pregnant women in Taiwan is 22%. Serotype III and VI are the two leading capsule types and the CC1 is the predominant clonal complex. Penicillin was still the first choice for treating GBS infection.

稿件檔: